Cell Imaging Multi-Mode Reader

Cell Imaging Multi-Mode Reader

Cytation™3 is a cell imaging multi-mode microplate reader that combines automated digital microscopy and conventional microplate detection. This patent pending design provides rich phenotypic cellular information with well-based intensity data.

Cytation™3 is a cell imaging multi-mode microplate reader that combines automated digital microscopy and conventional microplate detection.  This unique patent pending design provides rich phenotypic cellular information with well-based quantitative data. Equipped with BioTek’s patented Hybrid Technology™ for microplate detection, Cytation3 includes both high sensitivity filter-based detection and a flexible monochromator based system for unmatched versatility and performance. The upgradable fluorescence cellular imaging module provides researchers rich cellular visualization analysis without the complexity and expense of standard automated microscopes, making cell imaging more accessible to a larger number of research laboratories at an affordable price.

Fluorescence microscopy is a powerful technique for visualizing cellular responses to understand cell proliferation, protein expression, cytotoxicity and other expressions. The ability to perform both conventional quantitative fluorescence measurements and cell imaging takes cell biology research to another level.  With special emphasis on live-cell assays, Cytation3 features temperature control to 45 °C, CO2/O2 gas control, orbital shaking and full support for time lapse studies with BioTek’s Gen5™ software, specifically designed to make plate reading and image capture easy.

Technology advances are found throughout Cytation3’s including high-intensity LED light sources, filter cubes optimized for high signal/noise ratio, hard coated optical filters, Olympus objectives, and superior autofocus for totally software controlled microscopy.

Imaging mode Fluorescence and brightfield
Detection mode Monochromators: FL, Lum, UV-Vis Abs
Filters: FL, TRF, FP, Lum
Read method Endpoint, time-lapse, kinetic, well mode, montage
Labware type 6- to 384- well microplates, microscope slides, cell culture flasks (T25), Compatible with Take3™ Micro-Volume Plates with 2 µL microspots
Temperature control To 45 °C +0.5 °C at 37 °C
Shaking Linear, orbital, double orbital
Automation BioStack and 3rd party automation compatible
CO2 and O2 Control 0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller
Software Gen5™ Data Analysis Software included.
Imaging System
Light source High power LEDs
Camera 16-bit gray scale
Filter cube capacity 4 onboard, user-replaceable cubes
Objective capacity 2 onboard user-replaceable objectives
Available objectives 2x, 4x, 10x, 20x
Image collection rate 96 wells, 1 image/well, 20x, 8 minutes
Fluorescence Intensity
Sensitivity Monochromators:
Top: fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate)
Bottom: fluorescein 5 pM typical (0.5 fmol/well 384-well plate)

Fluorescein 1 pM typical (0.1 fmol/well 384-well plate)
Light Source Xenon Flash lamp
Wavelength Selection Double grating monochromators (Top and Bottom) and, Deep blocking bandpass filters / dichroic mirrors (Top)
Wavelength Range Monos: 250 - 700 nm
Filters: 200 - 700 nm (850 option)
Dynamic Range 5 decades
Detection System Two PMT detectors: one for monochromator system, one for filter system
Sensitivity Monochromator system: 20 amol ATP typical (flash)
Filter system: 10 amol ATP typical (flash)
Wavelength Range 300 - 700 nm
Dynamic Range >6 decades
Fluorescence Polarization
Sensitivity 5 mP at 1 nM fluorescein typical
Wavelength Range 320 - 700 nm (850 nm option)
Time-Resolved Fluorescence
Sensitivity Europium 100 fM typical with filters (10 amol/well in 384-well plate)
Europium 1200 fM typical with monos (120 amol/well in 384-well plate)
Light Source Xenon flash lamp
Wavelength Range Monos: 250 - 700 nm
Filters: 200 - 700 nm (850 option)
Light Source Xenon flash lamp
Wavelength Selection Monochromator
Wavelength Range 230 - 999 nm, 1 nm increment
Bandpass 4 nm (230-285 nm), 8 nm (>285 nm)
Dynamic Range 0 - 4.0 OD
Resolution 0.0001 OD
Reagent Dispensers
Number 2 syringe pumps
Dispense Volume 5 - 1000 µL in 1 µL increment
Dead Volume 1 mL, 100 µL with back flush
Plate Geometry 6- to 384-well microplates
Dispense Precision <2% at 50-200 µL
Dispense Accuracy +1 µL or 2%
Physical Characteristics
Power 130 Watts max.
Dimensions 20” D x 16.5” W x 17.5” H
(50.8 cm x 41.91 cm x 44.5 cm)
Weight 80 lbs (36.3 Kg)
Regulatory CE and TUV marked. Models for In Vitro Diagnostic use are available.
  • Automated digital microscopy and/or multi-mode microplate detection in one instrument
  • Modular and upgradable: microscopy only, microplate reading only, or both
  • Flexible sample format: 6- to 384-well plates, microscope slides and cell culture flasks
  • Affordable automation: automated XY stage, auto focus, auto exposure, automated image capture, LED intensity
  • Inverted fluorescence (4 LED filter cube capacity) and brightfield microscopy
  • From 2x for full-well imaging to 20x for sub-micron resolution for intra-cellular details
  • High quality images: Olympus objectives, 16-bit gray scale CCD camera, Semrock filters.
  • Live cell assays with temperature control up to 45 °C, CO2/O2 gas control accessory
  • Endpoint, time-lapse and montage assays
  • Automatic cell counting, sub-population analysis and image statistics
  • Dual reagent injectors
  • Patented Hybrid Technology™ microplate reading mode with high performance filter-based optics and high-flexibility monochromator optics

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.

BioStack3 Microplate Stacker

BioStack3 is the fastest stacker available to integrate with BioTek microplate instruments including washers, dispensers and readers. The new design allows compact, walk-away automation for benchtop or laboratory enclosure use.