Filter-based Multi-Mode Microplate Readers

Filter-based Multi-Mode Microplate Readers

Filter-based readers are the best compromise between performance, flexibility and cost. They are extremely efficient optically and deliver an unmatched level of sensitivity. Synergy HT is used in thousands of life science research laboratories worldwide for the most common microplate-based applications. Synergy 2, with its dichroic-based optical system, and fully modular architecture, is the reader of choice if you need the best level of performance.

The Synergy 2 is BioTek's high-performance multi-mode plate reader designed for the life science research and drug discovery markets. Its fluorescence detection system uses deep blocking filters and dichroic mirrors for the best level of performance in research and screening applications. The dedicated absorbance detection system is monochromator-based, providing the best level of flexibility in this read mode. And, by utilizing dedicated optical elements for each individual detection technique, Synergy 2 offers uncompromised performance and the lowest limits of detection.

Detection modes include Fluorescence Intensity, Fluorescence Polarization, Time-Resolved Fluorescence, AlphaScreen, Luminescence and UV-visible Absorbance. Its three broad-spectrum light sources have been chosen for optimal illumination and excitation in all applications.

Detection mode Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa®, Luminescence, UV-Visible Absorbance
Read method End point, kinetic, spectral scanning, well-area scanning
Microplate types 6- to 1536-well plates (luminescence 1-384)
PCR plates
Compatible with Take3 Multi-Volume Plate with 2 µL microspots
Temperature control 4°C above ambient to 65°C; +0.5°C at 37°C
Shaking Yes
Software Gen5™ included
Control through USB or serial port
Automation Yes
Light source Tungsten Halogen Lamp
Sensitivity 100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate
Dynamic range >6 decades
Reading speed 2 minutes typical for 96-well plate
Detection system Ultra Low Noise PMT
Light source SQ Xenon Flash
Wavelength selection Monochromator
Wavelength range 200 - 999 nm, 1 nm increment
Bandpass 2.4 nm
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2 OD typical
<3% at 3.0 OD typical
OD linearity <1% from 0 to 3.0 OD typical
OD repeatability <0.5% at 2.0 OD typical
Stray light 0.03% at 230 nm typical
Reading speed 96: 11 seconds
384: 22 seconds
1536: 42 seconds
Fluorescence Intensity
Sensitivity Fluorescein 1 pM typical (0.2 fmol/well 96-well plate; 0.1 fmol/well 384-well plate) - Top
Fluorescein 5 pM typical (1 fmol/well 96-well plate; 0.5 fmol/well 384-well plate) - Bottom
Light source Tungsten Halogen
High Energy DPR Xenon Flash (optional)
Wavelength selection Deep blocking bandpass filters/dichroic mirrors
Wavelength range 300 - 700 nm with tungsten lamp (850 nm option)
200 - 700 nm with xenon lamp (850 nm option)
Dynamic range >6 decades
Bandpass Filter dependent
Detection system PMT
Reading speed 96: 11 seconds
384: 22 seconds
1536: 42 seconds
Sensitivity 10 amol ATP typical (flash)
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Detection system Low noise PMT
Fluorescence Polarization
Light source Tungsten Halogen
High Energy DPR Xenon Flash (optional)
Sensitivity 3 mP at 1 nM fluorescein typical
Wavelength selection Deep blocking filters/mirrors
Wavelength range 400 - 700 nm (320 - 850 nm option)
Time-Resolved Fluorescence
Light source High Energy DPR Xenon Flash
Sensitivity Europium 60 fM typical (12 amol/well 96-well plate; 6 amol/well 384-well plate)
Wavelength selection Deep blocking filters/mirrors
Wavelength range 200 - 700 nm (850 nm option)
Reagent Dispensers
Number 2 syringe pumps
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1.1 mL, 100 µL with backflush
Physical Characteristics
Power 250 Watts max
Dimensions 17"W x 17.5"D x 14.5"H (43.5 x 44.5 x 37.3 cm)
Weight 60 lbs (27 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.
  • Measurement Techniques: Fluorescense Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, Glow and Flash Luminescence, UV-Vis Absorbance, FRET, TR-FRET, BRET, Area Scanning, Spectral Scanning 
  • Unique Optical System: a narrow band monochromator, optimized filter/dichroic mirror pairing, and three broad spectrum light sources achieve superior performance in all read modes
  • Compatible with Take3 Micro-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
  • High Sensitivity: dedicated optical elements optimized for each specific modality result in the lowest limits of detection available in one compact instrument
  • Modular Design: purchase only what you need and easily upgrade later as experimental requirements expand
  • High Throughput: optimized for screening assays including 1536-well read mode
  • Wide Range of Applications: with built-in temperature control, shaking, and optional dual reagent injector, virtually any microplate-based application is possible
  • High Performance Screening Assays: The Synergy 2 is used in screening laboratories with all the major assay technologies such as:

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.

BioStack3 Microplate Stacker

BioStack3 is the fastest stacker available to integrate with BioTek microplate instruments including washers, dispensers and readers. The new design allows compact, walk-away automation for benchtop or laboratory enclosure use.