Fluorescence Readers

Fluorescence Readers

BioTek offers a wide choice in fluorescence detection, from the low-cost, highly sensitive FLx800™ to the modular Synergy™, BioTek has the fluorescence reader you need. Cost effective filter-based systems and advanced monochromator-based designs are available. The unique patented Hybrid Technology, which combines monochromator and filter-based optics, is available on several Synergy models, providing incredible performance and application flexibility.

The FLx800™ Multi-Detection Microplate Reader combines excellent specifications and performance with convenience and ease of use. This design incorporates powerful performance at a price much lower than traditional fluorescence-luminescence microplate readers. The FLx800 line includes several models with options that meet the specific needs of research and OEM users. Top and bottom reading are available to read from 6-well to 384-well microplates. An optional external dispenser module allows you to run applications such as ion channel assays and flash luminescence assays (e.g. luciferase, ATP).

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.

Detection mode Fluorescence, Luminescence
Read method End point, kinetic and area scanning under computer control
Microplate types 6- to 384-well plates
Temperature control 4°C above ambient to 50°C (I models)
Shaking Yes (I models)
Software Gen5 Data Analysis Software
Automation No
Onboard Software 55 user-programmable protocols
Fluorescence Intensity
Sensitivity Fluorescein 5 pM typical (1 fmol/well 96-well plate)
Light source Tungsten Halogen
Wavelength selection Deep blocking filters
Wavelength range 300 - 700 nm
Dynamic range 5 decades
Sensitivity 100 amol ATP typical (flash)
Wavelength range 300 - 700 nm
Dynamic range 5 decades
Reagent Dispensers
Number 1 syringe pump
Dispense volume 5 - 1000 µL in 1 µL increments
Dead volume 1.1 mL, 100 µL with backflush
Physical Characteristics
Power 100 - 240 VAC 50/60 Hz
Dimensions 15"W x 16"D x 9"H (38.1 x 40.64 x 22.89 cm)
Weight 30 lbs (13.6 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.
  • Custom-mapped fiber optics for optimal signal transfer
  • Selectable, automated top- or bottom-probe reading for best sensitivity
  • Typical fluorescence sensitivity to 5 pM sodium fluorescein
  • Glow luminescence reading with top detection for best sensitivity
  • Incubation to 50ºC for temperature-sensitive assays
  • Reads 6- to 384-well plates
  • Convenient front panel accessory access makes changing filters simple
  • Powerful on-board data reduction software
  • Computer control via Gen5™ Software