This section introduces components for the design of application specific microfluidic systems. Micro casting of PDMS (Polydimethylsiloxane) provides optical transparent flow-through cells (MicCell). GeSiM offers complete kits for home made PDMS cells.
Our PDMS casting station enables home made microfluidic cells. Explore the possibilities of the microscope tool MicCell . Coated silicon wafers have proofen as masters for PDMS casting. Robustness is a main benefit of the compact hydrogel valves. The flow sensors measure flows up to 70 microliter per minute. They come with standard fittings. If dead volume matters they are also amenable for integration into microfluidic systems. Macrofluidic components as well as software enables the convenient supply of microfluidic flow through cells.
In order to provide a microfluidic system with maximum flexibility, GeSiM has developed flowthrough cells with removable coverslip that are assembled in seconds and can be used for numerous applications including live cell imaging and single molecule detection.
MicCell is a platform technology to combine micro and macro fluidic components, lab-on-a-chip devices, microscopy methods, analytical methods and imaging technologies. MicCell allows to work interactive or fully automatically with all the components in a customized approach. The core component of MicCell is the micro flowthrough cell, typically made of polydimethylsiloxane (PDMS) and glass. GeSiM offers a special PDMS-based channel system and provides this as PDMS-Channel-Plate (PDMS-CP). Depending on users demand our PDMS-CP concept is suitable as a disposable or a multi-use product.
- Simple and quick assembly
- Maximum flexibility by modular design
- Gentle handling of cells, beads and biomolecules
- Complete fluid control by external syringe pumps
We manufacture masters for hot embossing for the mass production microfluidic components from materials like PMMA and Topas®. Masters consist of silicon that is anisotropically etched in SF6-O2 plasma (advanced silicon etching, ASE) and then coated by a Teflon-like material. GeSiM has optimized the ASE procedure with respect to wall angle and smoothness, and in conjunction with the Teflon-coating master tools are produced that can be easily demolded.
Hydrogel Micro Valves
Our patented microvalve has no membranes and is therefore extremely reliable and robust. It works because the polymer particles captured in a small cage dramatically change their size (by more than a factor of 10!) with temperature. Our standard valves feature a biocompatible switching temperature of 34 °C.
- Various housings available. Death volume of the whole device approximately 5 micro liters.
- Hydrogel actuator is formed by polymer particles, captured in a micro-basket of (800 x 800 x 200) µm³.
- Dependent on the temperature, the polymer is shrinking or swelling.
- Phase transition temperature TT is adjustable. In the hydro gel used in GeSiM valves TT = 33.8°C.
- To improve dynamics, the working temperature of actuator is adjusted to 28°C.
- Normally closed hydrogel valves are available.
- Heating power to actuate the valve is in maximum 250 mW, the applied voltage is between 3.5 V and 5 V.
The GeSiM microfluidic flow sensor measures small flow rates, such as those in microfluidic systems. It features a thermocalorimetric measurement. The liquid is heated up slightly, the temperature profile is analyzed and digitalized by an integrated A/D-converter. The measurement range starts at less than one µl/min and goes up to approx. 100 ul/min. The flowmeter connects to a PC through a serial interface (RS232) and comes with a WindowsTM based measurement software.
The micro flowmeter comprises cascadable sensor modules and controller modules. It allows the easy setup of multifold sensor arrays with one up to sixteen sensors (In any combination). Different tubes and tube adapters provide a quick integration into your system setup.
The control software supports the linearization of sensor characteristics, the display of the measured curves in one or more fully configurable diagrams and the storing of the data in spreadsheet-compatible formats. And all that for a surprisingly low price.