Monochromator-based Multi-Mode Microplate Readers

Monochromator systems provide ease of use, flexibility and cover a broad range of applications out of the box. Synergy H1m is the best value monochromator-based multi-mode reader on the market, while Synergy Mx is the best choice if you need advanced features and performance.

Monochromator-based Multi-Mode Microplate Readers

Ultra Fine-Tuned™ technology is what sets the Synergy Mx Multi-Mode Reader apart. Its quadruple monochromator system selects wavelengths with a repeatability of plus or minus 0.2 nm. Its top optical head can focus up and down on the samples with a 100 µm resolution. Four slits on the excitation and emission side provide a choice of 16 bandpass combinations for every wavelength pair. Its advanced 4-Zone™ temperature control system incubates up to 65°C with a precision of ± 0.5°C at 37°C. These unique features and BioTek’s exclusive focus on microplate instrumentation and software make the Synergy Mx the most precise, sensitive and flexible multi-mode microplate reader available today for life-science research applications.

Gen5 Data Analysis Software

BioTek’s new Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5's data reduction, will be evident in increased laboratory efficiency.

BioStack3 Microplate Stacker

BioStack3 is the fastest stacker available to integrate with BioTek microplate instruments including washers, dispensers and readers. The new design allows compact, walk-away automation for benchtop or laboratory enclosure use.

Detection mode Fluorescence, UV-Visible Absorbance, Luminescence, Time-Resolved Fluorescence (secondary mode)
Read method End point, kinetic, spectral scanning, well-area scanning
Microplate types 1- to 384-well plates and PCR plates
Compatible with Take3 Multi-Volume Plate with 2 µL microspots
Temperature control 4°C above ambient to 65°C +0.5°C at 37°C
Shaking Yes
Software Gen5™ included
Control through USB or serial port
Automation Yes
Light source High Energy Xenon Flash
Wavelength selection Double Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Bandpass 2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 2.0 OD typical
OD linearity <1% from 0 to 3.0 OD typical
OD repeatability <0.5% at 2.0 OD typical
Reading speed 96 wells: 11 seconds
384 wells: 22 seconds
Fluorescence Intensity
Sensitivity Fluorescein 2 pM typical (0.2 fmol/well 384-well plate) (Top)
Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) (Bottom)
Light source High Energy Xenon Flash
Wavelength selection Double grating monochromators (Top and Bottom)
Wavelength range 250-850 nm
Dynamic range 5 decades
Bandpass Variable: 9, 13.5, 17, 20 nm
Detection system Dedicated red shifted PMT
Reading speed 96: 11 seconds
384: 22 seconds
Sensitivity 10 amol ATP typical (flash)
Wavelength range 300 - 700 nm
Dynamic range >6 decades
Detection system Low noise PMT
Reagent Dispensers
Number 2 syringe pumps
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1.1 mL, 100 µL with back flush
Physical Characteristics
Power 250 Watts max
Dimensions 17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm)
Weight 70 lbs (31.5 kg)
Regulatory CE and TUV marked. In Vitro Diagnostic use models are available.
  • Fully monochromator-based with quadruple grating architecture for Ultra Fine-Tuned performance.
  • Compatible with Take3 Micro-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.
  • High performance fluorescence (top and bottom), absorbance and dedicated luminescence detection systems accommodate all life-science research microplate applications.
  • Variable bandpass selection adds flexibility in fluorescence mode.
  • Automated Z-height adjustment accommodates variable microplate heights and liquid levels with 100 μm resolution.
  • Cuvette port to measure standard 1-cm path cuvettes if only a few samples need to be run, or to compare data with a standard cuvette spectrophotometer.
  • Modular architecture allows purchase of required modes with the ability to upgrade later.
  • Optional dual-reagent dispenser can be used for assays requiring precise timing such as ion channel assays or luminescent flash ATP assays.